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KMID : 1007520090180020570
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Food Science and Biotechnology
2009 Volume.18 No. 2 p.570 ~ p.573
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Enzymatic Preparation of Maltooctaose-rich Mixture from Starch Using a Debranching Enzyme of Nostoc punctiforme
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Choi Ji-Hye
Kim Myo-Jeong
Kim Young-Wan
Lee Hee-Seob
Park Jong-Tae
Lee Byong-Hoon
Park Kwan-Hwa
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Abstract
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The debranching enzyme of Nostoc punctiforme (NPDE) is a novel enzyme that catalyzes the hydrolysis of α-1,6-glycosidic linkages in starch, followed by the sequential hydrolysis of α-1,4-glycosidic linkages. The debranching activity ofNPDE is highly specific for branched chains with a degree of polymerization (DP)>8. Moreover, the rate of hydrolysis of α-1,4-linkages by NPDE is greatly enhanced for maltooligosaccharides (MOs) with a DP>8. An analysis of reaction mixturescontaining various starches revealed the accumulation of maltooctaose (G8) with glucose and maltose. Based on the novelenzymatic properties of NPDE, an MO mixture containing more than 60% G8 with yield of 18 g G8 for 100g starch wasprepared by the reaction of NPDE with soluble starch, followed by ethanol precipitation and gel permeation chromatography(GPC). The yield of the G8-rich mixture was significantly improved by the addition of isoamylase. In summary, a 4-stepprocess for the production of a G8-rich mixture was developed involving the enzymatic hydrolysis of starch by NPDE.
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KEYWORD
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Nostoc punctiforme, cyanobacteria, debranching enzyme, maltooctaose-rich mixture, maltooligosaccharide
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